Hemoglobin biosynthesis in individual bursts in culture: studies of human umbilical cord blood

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Hemoglobin biosynthesis in individual bursts in culture: studies of human umbilical cord blood.

We cultured human umbilical cord blood erythropoietic precursors in methyl cellulose clonal assay and analyzed the synthetic rates of Hb A and Hb F in individual erythropoietic bursts. Hemoglobin was labeled with 14C-amino acids in culture, separated by slab gel isoelectric focusing techniques, and quantitated by fluorographic methods. Almost all bursts exhibited both Hb A and Hb F in varying r...

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comparing mean hemoglobin levels of umbilical cord blood in neonates with maternal hemoglobin and delivery pattern

introduction hemoglobin (hb) levels in fetus increase with progression of pregnancy, reaching highest levels in life. hb serves as iron reserve in fetus and is needed for the infant to adapt with anemia. many factors can decrease hb levels at birth and lead to accelerated physiological anemia. hence, the infant would require earlier administration of iron drops. identification of these factors ...

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A Review of Procedures Involved in Human Umbilical Cord Blood Banking and Transplantation

Cord blood hematopoietic stem cells are widely used as an alternative source for hematopoietic stem cells transplant. Increasing rate of patients who need hematopoietic stem cells transplant and many advantages of cord blood in comparison to bone marrow hematopoietic stem cells, have promoted banking of cord blood units. Cord blood banking requires accurate steps in donor selection, cord blood ...

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The Correlation between Type of Delivery and Umbilical Cord Blood Hemoglobin and Hematocrit in Full- Term Neonates

Introduction: Neonates with lower hemoglobin and Hematocrit are more at risk of iron deficiency anemia. This rate is influenced by several factors. Children's health can be improved by identifying these factors and taking appropriate actions. This study determined the relationship between type of delivery and umbilical cord blood hemoglobin and Hematocrit. Methods: This descriptive correlation...

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Composition of ‘ y - Chains in the Hemolysates of an Umbilical Cord Blood and Erythropoietic Bursts From Carriers for Hemoglobin

By using a globin isoelectric focusing technique. we measured the levels of G’y. Malta-I G’y, and ky globin chains in the hemolysates of an umbilical cord blood of a carrier for hemoglobin F Malta-I (“yl 1 7 HisArg). a G’y variant. Malta-I G’y focused slightly cathodal to $-chains. Malta-I G’y. normal G’y. and ky chains comprised 21.8%. 57.5%. and 20.7% of the total ‘y-chains, respectively. Thu...

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ژورنال

عنوان ژورنال: Blood

سال: 1979

ISSN: 0006-4971,1528-0020

DOI: 10.1182/blood.v53.3.519.bloodjournal533519